Light enhanced the antimicrobial, anticancer, and catalytic activities of selenium nanoparticles fabricated by endophytic fungal strain, Penicillium crustosum EP-1

Selenium nanoparticles (Se-NPs) has recently received great attention over owing to their superior optical properties and wide biological and biomedical applications. Herein, crystallographic and dispersed spherical Se-NPs were green synthesized using endophytic fungal strain, Penicillium crustosum EP-1. The antimicrobial, anticancer, and catalytic activities of biosynthesized Se-NPs were investigated under dark and light (using Halogen tungsten lamp, 100 Watt, λ > 420 nm, and light intensity of 2.87 W m−2) conditions. The effect of Se-NPs was dose dependent and higher activities against Gram-positive and Gram-negative bacteria as well different Candida spp. were attained in the presence of light than obtained under dark conditions. Moreover, the viabilities of two cancer cells (T47D and HepG2) were highly decreased from 95.8 ± 2.9% and 93.4 ± 3.2% in dark than those of 84.8 ± 2.9% and 46.4 ± 3.3% under light-irradiation conditions, respectively. Significant decreases in IC50 values of Se-NPs against T47D and HepG2 were obtained at 109.1 ± 3.8 and 70.4 ± 2.5 µg mL−1, respectively in dark conditions than 19.7 ± 7.2 and 4.8 ± 4.2 µg mL−1, respectively after exposure to light-irradiation. The photoluminescence activity of Se-NPs revealed methylene blue degradation efficiency of 89.1 ± 2.1% after 210 min under UV-irradiation compared to 59.7 ± 0.2% and 68.1 ± 1.03% in dark and light conditions, respectively. Moreover, superior stability and efficient MB degradation efficiency were successfully achieved for at least five cycles.


Results and discussion
Green synthesis of Se-NPs. In the current study, Se-NPs were fabricated by harnessing the active metabolites secreted by the endophytic fungal strain, Penicillium crustosum EP-1 for reducing and capping the Na 2 SeO 3 to form Se-NPs. At the first, the successful fabrication was confirmed by the color change of fungal biomass filtrate after mixing with metal precursor from colorless to ruby red color. The formed color was gradually increased with time as a result of SeO 3 reduction; therefore, we checked the formed color and measured its absorbance after 24 h of incubation. After that, no further color change was found and this depicts the complete reduction of SeO 3 to Se 0 . Compatible with this finding, the absorbance of NPs with times was increased but did not shift the SPR peak, this is due to the complete reduction of metal ions to metal NPs and hence increasing the color intensity as reported previously [28][29][30] . The color of Se-NPs synthesized by Trichoderma atroviride was completely formed after incubation of fungal biomass filtrate with a metal precursor for 24 h 31 . In a recent study, the extracellular metabolites secreted by P. chrysogenum strain F9 isolated from historical manuscript were used as a biocatalyst to reduce selenium ions forming Se-NPs 8 . The synthesis conditions especially pH values exhibited a critical role in the green synthesis process. Herein, the synthesis of Se-NPs was better in an alkaline medium (pH = 8) because various functional groups in fungal biomass filtrate that are responsible for reduction and capping were more active in alkaline conditions. Also, the alkaline medium prevents the agglomeration of www.nature.com/scientificreports/ NPs as well as stabilize the capping agents on the NPs surfaces via reacting with amino groups on the protein surfaces 32,33 . The green synthesis of NPs using fungi has gained more attention than other microorganisms. This is mainly due to the exceptional secretion of extracellular metabolites that increase the NPs yield and impart high stability to the synthesized NPs 34 . Also, fungi are characterized by easy handling, high metal tolerance, high biomass production, and high scalability 35 . The genus Penicillium is composed of approximately 354 species that are characterized by producing a wide range of metabolites 36 . In this study, the endophytic strain P. crustosum EP-1 was selected for Se-NPs production because of its high efficacy in producing a wide variety of metabolites including enzymes (amylase, cellulase, gelatinase, and xylanase), bioactive metabolites as previously reported 37 . Therefore, we predicted the potentiality of this strain to form small size and highly stable Se-NPs.
Characterizations of Se-NPs. The first monitor for the successful formation of Se-NPs is the ruby red color that is substantiated by measuring its absorbance using UV-Vis spectra. Data showed that the high absorption peak was at 270 nm ( Fig. 1A) and this peak formed and characterized the SPR of Se-NPs. The wide SPR peak mainly reveals the polydispersity of green synthesized Se-NPs. Compatible with our study, the SPR of Se-NPs synthesized by bacterial strain Ralstonia eutropha was at 270 nm 30 . The maximum SPR peak of Se-NPs synthesized by aqueous extract of Ceropegia bulbosa was observed at 277.5 nm 25 . Recently, the water extract of Cirsium setidens was changed from yellow green to brick red color after mixing with Na 2 O 3 Se indicates the formation of Se-NPs that exhibit maximum absorption peak at 278 nm which is correlated to SPR of Se-NPs 38 . Various researchers have reported that the SPR peak in the ranges of 200-300 nm is related to the surface plasmon vibration of Se-NPs 31,39 . The roles of different functional groups that exist in the fungal biomass filtrate in the reduction and stabilization of Se-NPs were demonstrated through a comparison of the FT-IR spectra (Fig. 1C). The peak at 3433 cm −1 was assigned to O-H and N-H overlapping vibration 41 , this peak was shifted to 3441 cm −1 with the same intensity as Se 0 was fabricated. The broadband at 2078 cm −1 of the biomass filtrate was assigned mainly to the sulfonic acid groups (from the medium) and in the carbohydrate moiety (verifying by EDX analysis). The peaks at 1633 cm −1 and 1634 cm −1 were assigned to the C=O overlapped with the N-H stretching vibration of the produced polysaccharides. Extra peaks that appeared as Se manufactured including 2424 cm −1 for S-H stretching bands, and 1382 cm −1 for deformation of NO 3 stretching, 1100 cm −1 for C-O-C asymmetric stretching and C-N stretching, while 833 cm −1 for C-O-S stretching overlapped with nitrate ions 42 .
There are several parameters determining the incorporation and activities of NPs in specific biomedical and biotechnological applications. These include shapes, coating agents, sizes, elemental compositions, solubility, zeta potential, and aggregation values 43,44 . Therefore, TEM, SEM, and EDX analyses were investigated for the bio-fabricated Se-NPs. As shown in Fig. 2A,B, the green synthesized Se-NPs were a spherical shape, well dispersed, without aggregation and agglomeration, and have a sizes range of 3-22 nm with an average particle size of 9.11 ± 3.56 nm.
The activity of Se-NPs is mainly dependent on their size and always exhibits increment with smaller sizes. For example, the antioxidant activity of Se-NPs fabricated by Pantoea agglomerans showed a promising result for a smaller size than the large one 45 . Moreover, Se-NPs synthesized by aqueous extract of garlic showed high antimicrobial activity with sizes of 21-40 nm than the activity showed by sizes of 41-50 nm 46 . Due to obtained small Se-NPs sizes in the current study, it can be integrated into various activities.
The morphology and chemical compositions of green synthesized Se-NPs, were analyzed by SEM-EDX (Fig. 2C,D). As shown the absorption peaks of Se were identified at 1.38 keV, 11.24 keV, and 12.41 keV for SeLα, SeKα, and SeKβ, respectively. The obtained data are compatible with those reported that the characteristic absorption peaks of Se-NPs synthesized by aqueous extract of Vitis vinifera were at 1.37 keV, 11.22 keV, and 12.49 keV for SeLα, SeKα, and SeKβ, respectively 47 . Also, the absorption peaks of Se-NPs fabricated by the bacterial strain www.nature.com/scientificreports/ Bacillus cereus were observed at 1.37 keV, 11.22 keV, and 12.49 keV for SeLα, SeKα, and SeKβ, respectively 9 . The EDX chart showed that the Se was represented by the highest weight element in the sample with percentages of 42.28 and atomic percentages of 38.39 (Fig. 2D). Other weak peaks such as S and Cl were represented in the EDX chart with weight percentages of 0.44 and 5.76%, respectively. These peaks can be related to glass underlay as reported previously 10 . In addition, peaks of C, O, P, and K were present with weight and atomic percentages of (16.82, 14.04, 0.72, and 19.94%) and (32.01, 14.8, 0.49, and 10.21%), respectively. These signals can be originated from fungal biomolecules such as enzymes and proteins that cap and impart the stability of Se-NPs 48 .
Antimicrobial activity of Se-NPs. Figures 25 . Also, the inhibitory effects of Se-NPs fabricated by lactic acid bacteria, Lactobacillus paracasei against different species of Candida and Fusarium were increased by increasing the concentrations 49 . Moreover, Se-NPs synthesized by Bacillus subtilis exhibited high antibacterial and antifungal activity in a dose-dependent manner 50 .
To implicate the green synthesized Se-NPs in the pharmaceutical industries, the minimum inhibitory concentration (MIC) values for each tested organism should be determined. The activity of different concentrations Overall, our study has shown that the fungal species of Candida were more sensitive toward green synthesized Se-NPs than bacterial strains. Among prokaryotic strains, the Gram-positive bacteria were more sensitive than Gram-negative bacteria. The obtained data are compatible with those reported for the Phyto-fabricated Se-NPs using fruit aqueous extract of Emblica officinalis that exhibited higher antimicrobial activity against fungi than Gram-positive and Gram-negative bacteria 51 . The NPs size is considered the main factor for their inhibitory effect. The smaller size should easier penetrate the microbial cell wall and cell membrane and hence induces the lysis of microbial cells 52 . Moreover, it can effortlessly interfere with microbial metabolic activities more than larger size NPs. Among these activities, ATP synthesis, respiratory sequence, cell division, and transport chains which ultimately accelerate cell death 51,53 . Therefore, the small Se-NPs size (3-22 nm) formed in the current study is potential responsible for the wide range of antimicrobial activity.
Our study proved that the synthesized Se-NPs have higher antimicrobial activity against Gram-positive than Gram-negative bacterial strains. This may be attributed to the high electrostatic repulsion of synthesized Se-NPs toward negatively charged membrane components and lipopolysaccharide in Gram-negative bacteria. Whereas negative charges are less in Gram-positive bacteria and hence Se-NPs are highly deposited on the surface of Gram-positive bacteria leading to cell death 54,55 . Candida especially C. albicans are considered the main opportunistic yeast that causes mild to acute diseases in the oral cavity, skin, gastrointestinal, mucous membranes, and urogenital tracts in the low immunity persons, pregnant women, and persons having antibiotic resistance due to misuse for long times 56 . Our study has shown that the synthesized Se-NPs have promising activity against different species of Candida. This activity can be related to the efficacy of Se-NPs to inhibit the ergosterol pathways that www.nature.com/scientificreports/ lead to alteration of sterol profile in the cell wall of Candida spp. 57,58 . Also, Se-NPs can be highly accumulated in the Candida cell wall by chemisorption, leading to the mixing of the cell wall proteins with selenium and hence destroying the amino acid containing sulfur such as cysteine and methionine 59 . The obtained data showed that the antimicrobial activity of green synthesized Se-NPs was enhanced in the presence of light, and this might be attributed to the production of various toxic free radicals such as O 2 ·− , · OH, and H 2 O 2 upon exposure to light that enhance the stresses in the cells as shown in Fig. 5.
Anticancer activity of Se-NPs. The MTT assay is used as a sensitive method to evaluate the cytotoxic effect of Se-NPs against cancer cell lines in the presence and absence of light irradiations. Data represented in Fig. 6 exhibited that the cell viability of cancer cells was dependent on the NPs concentration and the incubation conditions. Cell viability was decreased by increasing the Se-NPs concentration under light incubation than that   70,45, and 25% after exposure to various concentrations of Se-NPs at 20, 60, and 100 µg mL −1 , respectively, whereas these values were more decreased in presence of X-ray to be 45, 18, and 5%, respectively 10 .
In the current study, the microscopic examination of the treated cells showed that the monolayer sheet that characterized the epithelial cells were completely or partially lost at high concentration. Other symptoms such as buoyancy, shrinking, a tendency to be spherical or grainy and reduced cell population were also observed (Fig. 6A,B). These symptoms were more noticed in the presence of lights. The cytotoxic effect of NPs can be attributed to their size, smaller sizes are easy to penetrate the cell wall and hence interact with cell components leading to damage of main components such as DNA, proteins, and amino acids 63 . Moreover, light may have a synergistic effect on Se-NPs in enhancing the killing of cancer cells via apoptosis and/or cell arresting mechanisms. Increasing cell killing in presence of light irradiation is attributed to the efficacy of light to generate a high amount of reactive oxygen species (ROS) which harm mitochondria and hence initiate the apoptotic pathway.
Higher activity of Se-NPs was achieved in the presence of light irradiation. Analysis of variance showed that the IC 50 values were significantly decreased from 109.1 ± 3.8 µg mL −1 for T47D cells under dark conditions to 19.7 ± 7.2 µg mL −1 under light condition. Whereas the IC 50 values of Se-NPs against HepG2 cells were 70.4 ± 2.5 µg mL −1 and 4.8 ± 4.2 µg mL −1 under dark and light irradiation, respectively. The IC 50 results revealed that the activity of green synthesized Se-NPs as anticancer agents was higher in the HepG2 cell lines compared to T47D. This finding can be attributed to the high expression of purinoceptor in HepG2 cancer cells after treatment of Se-NPs. Compatible with the obtained data, the anticancer activity of ATP-Se-NPs in Caco-2 cell lines was higher than HEK293 cell lines because of high expression of purinoceptor in Caco-2 cells 64 . The obtained data confirmed low values of IC 50 and the high activity of low doses of Se-NPs in killing cancer cells under light irradiations would be more beneficial from these findings in chemo-and radiotherapeutic for regulating the progression of cancer cells. conditions. Data analysis showed that MB degradation was dependent on Se-NPs dose and contact time. The sorption or catalytic property was achieved on the nano-catalysts surface, the large surface area implies the high sorption activity 65,66 . In the current study, the green synthesized Se-NPs showed a promising catalytic activity due to their small size with large surface area. As shown in Fig. 7, 40.8 ± 0.9%, 47.6 ± 0.8%, and 52.05 ± 0.9% of MB were only degraded under dark conditions after 30 min with 0.2, 0.3, and 0.5 mg mL −1 of Se-NPs, respectively as compared with control (8.5 ± 1.9%). The dye degradation was increased gradually by increasing times to reach up to 57.3 ± 2.8%, 59.5 ± 0.9%, and 59.7 ± 0.2% at 0.2, 0.3, and 0.5 mg mL −1 Se-NPs, respectively after 210 min (Fig. 7) as compared to the physical degradation without Se-NPs treatment that reached to 9.4 ± 1.9% after 210 min. The obtained data are incompatible with those reported that the fuchsin basic dye did not exhibit any adsorption property after treatment with Se-NPs in dark conditions 62 .
Analysis of variance showed that the degradation efficiency of MB using Se-NPs was increased under light and UV-irradiation conditions. After 30 min of light irradiation, 45.9 ± 0.2%, 47.7 ± 1.5%, and 50.3 ± 0.8% of MB dyes were only decomposed using 0.2, 0.3, and 0.5 mg mL −1 Se-NPs, respectively (Fig. 7). The degradation percentages were gradually increased with times to reach 56.1 ± 0.4%, 66.4 ± 1.9%, and 68.1 ± 1.03% after 210 min using 0.2, 0.3, and 0.5 mg mL −1 Se-NPs, respectively. Interestingly, the highest degradation of MB dye was accomplished under UV-irradiation conditions (p ≤ 0.001). Dye degradation percentages of 65.1 ± 1.1% and 68.6 ± 2.7% were obtained after 180 and 210 min, respectively in the presence of UV-irradiation using 0.2 mg mL −1 Se-NPs. These percentages were increased up to 84.9 ± 0.5% and 89.1 ± 2.1%, respectively using 0.5 mg mL −1 Se-NPs (Fig. 7). The highest degradation percentages were attained with high amount of nano-catalyst because of increasing the adsorption sites number that exists on the nano-surface or high production of radicals that have a critical role in the degradation of the pollutants 67,68 . The electrostatic attraction of dyes toward NPs is mainly attributed to negatively charged dyes and positively charged NPs 69 .

Sorption of MB on Se-NPs surface.
The FT-IR and EDX were performed for the Se-NPs collected from the optimal MB photocatalytic experiment in presence of UV irradiation after 210 min to confirm the successful sorption of dye over the surface of the catalyst (Fig. 8). Appearance the series of new peaks mainly in the region of figure print, as well as decreasing the intensity of others that were mainly used in sorption was detected as the sorption of MB was performed (Fig. 8A). The OH and N-H stretching bands were decreased with shifts to 3418 cm −1 the new peak at 3030 cm −1 (associated with N + of the MB moiety) emphasizing the sorption of MB dyes (Fig. 8A) 70 . The peak at 2691 cm −1 is related to the S-H stretching band from the metabolite and the broad- www.nature.com/scientificreports/ band at 2134 cm −1 for the sulfonic groups 71 . The strong shift of the C=O peak of the Se-NPs composite (appeared at 1634 cm −1 ) to 1558 cm −1 is strong evidence for MB sorption. Peaks at 1488 cm −1 and 1445 cm −1 are assigned to ammonium groups for the MB 72 . The peak at 1382 cm −1 (for Se-NPs) is shifted to 1336 cm −1 , while other peaks at 1311 cm −1 are associated with a peak at 1336 cm −1 for the quaternary ammonium group for the MB 72 . Peaks at 1247 cm −1 , 1214 cm −1 , and 1157 cm −1 are related to C-N of tertiary and quaternary amines (derived from MB) moiety. The peaks at 944 cm −1 , 882 cm −1 , and 813 cm −1 for C-H of aromatic out-of-plane bend from the MB overlapped with C-O-S bands, while the peak at 774 cm −1 for OH out-of-plane bend from polysaccharide moiety. Peaks at 660 cm −1 and 615 cm −1 for C-S stretching 42,73,74 , and that at 532 cm −1 and 443 cm −1 for the aryl sulfides 75 . These data provide strong evidence for the adsorption of MB on Se-NPs.
Compatible with FT-IR, the presence of new peaks or change in the weight and atomic percentages of some peaks compared with the original EDX chart of Se-NPs indicates the successful sorption of MB (Fig. 8B). The presence of new peaks such as N with weight and atomic percentages of 2.67 and 3.28% may be originated from MB. Moreover, the increase in weight and atomic percentages of C and Cl peaks from (16.82 and 32.01%) and (5.76% and 3.81%) to (38.68% and 54.08%) and (7.27% and 6.02%), respectively confirmed the successful adsorption of MB over the catalyst. Also, increasing the weight and atomic percentages compared with the original EDX chart indicates the presence of MB on the surface of the catalyst (Fig. 8B).
The dye's decolorization and degradation using NPs can be explained by three mechanisms. The first mechanism is the transformation of colored dyes to leuco or white forms (colorless) due to the action of heat (thermochromism), light (photochromism), or pH of the medium (halochromism) 76 . This transformation can be achieved using an oxidation/reduction reaction. The synthesized Se-NPs interact with MB and generate electrons that reduce the central ring of the MB and hence change its conjugative structure to leuco form (colorless) as www.nature.com/scientificreports/ previously reported 77 . The second mechanism involves the sorption of dyes on the adsorption sites that exist on the NP's surface 78 . The smaller the size of Se-NPs synthesized in the current study, the larger their surface area, and hence high amounts of dyes can be adsorbed. This mechanism would be mainly used for dye removal under dark conditions. The third mechanism is the decomposition of dyes adsorbed on the surface of NPs due to the electron excitation by light exposure (photocatalyst). Accordingly, the higher activity of synthesized Se-NPs (antimicrobial and photocatalyst) in the presence of light has been achieved. Once the light hits the synthesized Se-NP, the photons of light will be absorbed over the surface of Se-NP that act as a photoactive center for producing the electron-hole pairs. As a results, the electrons are excited and transferred from valance band to conductance band of Se-NPs leaving holes in valance band. After that, the holes (h + ) in valence band interact with H 2 O forming high amounts of hydroxyl ( · OH) radicals. On the other hand, the excited electrons (e − ) in the conductance band interact with oxygen forming superoxide ( · O 2 − ) and hydrogen peroxide ( · OOH) radicals. In the end, the generated radical species react with MB leading to the degradation of CO 2 , H 2 O, and other degradable products as shown in Fig. 9 23 .
Reusability of green synthesized Se-NPs. The reusability of synthesized NPs in catalytic activity is considered an important factor for industrial applications 79 . Therefore, five sequential cycles were conducted to investigate the efficacy of synthesized Se-NPs. The reusability of Se-NPs was checked under the optimum conditions according to catalytic activity using 0.5 mg mL −1 after 210 min under UV-irradiation conditions. At the end of each cycle, the catalytic powder was collected and washed thrice with dH 2 O before being oven-dried at 60 °C for reuse in the subsequent cycle. Data analysis showed the superior stability of Se-NPs in degradation efficiency at different cycles with limited loss in the activity (Fig. 10). Analysis of variance showed that the degradation percentages of MB after the first cycle was 88.1 ± 2.5%. By repeating the photocatalytic activity, almost comparable degradation activities were achieved that decreased by only 4% after five cycles achieving degradation efficiency at 84.1 ± 0.9% (Fig. 10). ZnO-NPs, CuO-NPs, and their composites fabricated by Penicillium corylophilum also showed high stability in MB degradation after subsequential ten cycles with loss percentages of 4% 80 .   Endophytic fungal strain. The green synthesis of Se-NPs was achieved using the endophytic fungal strain, Penicillium crustosum EP-1. This strain was previously isolated from sterilized leaves of the medicinal plant Ephedra pachyclada 37 . The identification of this endophytic fungal strain was achieved by amplification and sequencing of the ITS gene and the obtained sequence was deposited in GenBank under the accession number MN954764.
Green synthesis of Se-NNPs. Heavy growth one disk (8 mm in diameter) of endophytic fungal strain EP-1 was cultured into sabouraud dextrose broth media (SDB) for 7 days at 30 ± 2 °C. After that, the inoculated SDB was filtrated using Whatman filter paper No. 1 to collect the supernatant (biomass filtrate) which was further subjected to centrifugation at 5000 rpm for 15 min to remove any adhering particles. The collected biomass filtrate was used as a reducing agent for the green synthesis of Se-NPs as follows: 51.9 mg of Na 2 SeO 3 was mixed well with 10 mL dH 2 O and added to 90 mL of collected biomass filtrate to get a final concentration of 3 mM. The previous mixture was mixed well under stirring conditions, at 38 °C, pH 8, for 1 h., and left overnight in dark conditions. The successful formation of Se-NPs was confirmed by color change to ruby red color. The biomass filtrate without metal precursor was running with the experiment as a control 81   www.nature.com/scientificreports/ in phosphate buffer) and incubated at 37 °C for 5 h in presence of 5% CO 2 . Finally, the final product (purple formazan crystals as MTT metabolic products) was dissolved in 10% DMSO. The plates were incubated in dark conditions under agitation for 30 min before measuring the intensity of formed color at 570 nm using ELIZA (Enzyme-Linked Immunosorbent Assay) microplate reader 10 . The cell viability after treatment with various concentrations of Se-NPs in the presence and absence of a light source was calculated according to the following equation: Degradation of dyes. The catalytic efficacy of synthesized Se-NPs was investigated through the degradation of methylene blue (MB) as a model dye. The experiment was achieved in dark, light (100-W, Halogen tungsten lamp), and UV (mercury lamp, λ > 253 nm) irradiation. The concentrations of Se-NPs used were 0.2, 0.3, and 0.5 mg mL −1 of MB solution (10 mg L −1 ). Before the experiment, the MB solution containing Se-NPs underwent stirring for 30 min to confirm the absorption/desorption equilibrium of dye on the catalyst surface 23 . After that, the mixture (MB solution and Se-NPs) was incubated in a dark condition under aeration. The previous step was repeated under the same conditions but with exposure to light and/or UV irradiation. At regular interval times, 2 mL of the treatment was withdrawn and centrifugated at 5000 rpm for 10 min. The optical density of clear supernatant was measured using an M-ETCAL spectrophotometer at maximum MB λ max (664 nm). The color removal percentages were calculated using the following equation 82 : where D, is the percentage of color removal; A, is the absorbance at zero-time; and B, is the final absorbance at interval times.
The reusability of Se-NPs as a catalyst for MB degradation was assessed under the optimum condition for five cycles. The catalyst was collected from the first cycle and washed thrice with dis. H 2 O and undergo oven-dry at 50 °C to remove the water content before being reused in the next cycle.

Statistical analysis.
All data in the current study are represented as the means of three independent replicates. Data were subjected to statistical analysis using the statistical package SPSS v17. The mean difference comparison between the treatments was analyzed by t-test or the analysis of variance (ANOVA) and subsequently by the Tukey HSD test at p < 0.05.

Conclusion
In this study, Se-NPs were green synthesized using the endophytic fungal strain, Penicillium crustosum. The crystallinity nature of as-formed Se-NPs was analyzed using XRD whereas the role of functional groups presents in the fungal extract to fabricate nano-Se was investigated by FT-IR. The shape and size of fungal-mediated Se-NPs were detected by TEM. The qualitative and quantitative composition of the sample was investigated by SEM-EDX. The photocatalytic performance of the biosynthesized Se-NPs exhibited higher activities including antimicrobial, anti-cancer, and the efficient degradation of dyes (methylene blue) than applications under dark conditions. Furthermore, Se-NPs as catalysts showed remarkable stability and reusability and that could be reused at least five times without remarkable loss of activity for degradation of organic dyes. From the obtained results, it can be concluded that the presence of light is one of the influential parameters to promote antimicrobial, anticancer, and catalytic activity of Se-NPs. The importance of this study has gained by the synthesis of Se-NPs through safe method using Penicillium crustosum as endophytic strain for the first time to resolve environmental and biomedical issues. Also, the comparative study between the activity of green synthesized Se-NPs in dark and light irradiation conditions will be more interesting for a wide range of researchers and readers.

Data availability
The data used to support the findings of this study are available from the corresponding author upon request. (1) Cell viability % = Absorbance of treated sample Absorbance of control × 100